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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Vitronectin Antibodies

          Invitrogen

          Vitronectin Monoclonal Antibody (A27)

          1 Published Figure
          2 References
          View all (13) Vitronectin antibodies

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          Cite Vitronectin Monoclonal Antibody (A27)

          • Published Figures (1)
          Vitronectin Antibody in Western Blot (WB)
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          Vitronectin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Vitronectin Antibody (CSI 004-27-02) in WB

          Scientific reports 2021 - Figure 4 Quantitative analysis of proteins in culture medium adsorbed by HA/CHA. ( a ) Western blots of Fibronectin, Vitronectin, and BSA remaining in culture medium after a 2 h incubation period in the presence of HA/CHA-covered monoliths with four HA/CHA microstructures. ( b ) Average normalized % adsorption of Fibronectin (blue), Vitronectin (red), and BSA (green) on HA/CHA-covered monoliths with four HA... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Vitronectin Antibody in Western Blot (WB)

          Product Details

          CSI 004-27-02

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          ELISA (ELISA)

          1:80,000
          -
          Product Specifications

          Species Reactivity

          Bovine, Sheep

          Published species

          Bovine, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          A27

          Immunogen

          Lysed bovine corneal endothelial cells and extracellular matrix.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.08 mg/mL

          Purification

          Protein A/G

          Storage buffer

          PBS, pH 7.4

          Contains

          15mM sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1088273

          Product Specific Information

          CSI 004-27-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 004-27-02 cross-reacts with sheep vitronectin, no reactivity with human or horse.

          CSI 004-27-02 has been successfully used in ELISA, Western blotting and Immunohistochemistry techniques. CSI 004-27-02 is suitable for ELISA, immunoblotting and immunostaining of frozen PLP-fixed sections of bovine tissues. The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.

          NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

          Target Information

          VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S-protein)

          View more View less

          Gene Aliases: BOS_18542

          View more View less

          Entrez Gene ID: (Sheep) 101119231, (Bovine) 507525

          View more View less

          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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